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FAQ
Posted on February 28, 2024
What is the structure of reverse transcriptase?
Cellular ProcessesDNA and RNA QuantitationNucleic AcidsNucleusReverse Transcription PCR (RT-PCR)
What is the structure of reverse transcriptase?
Cellular ProcessesDNA and RNA QuantitationNucleic AcidsNucleusReverse Transcription PCR (RT-PCR)
Posted on February 28, 2024
What organisms have reverse transcriptase?
Cellular ProcessesDNA and RNA QuantitationNucleic AcidsNucleusReverse Transcription PCR (RT-PCR)
What organisms have reverse transcriptase?
Cellular ProcessesDNA and RNA QuantitationNucleic AcidsNucleusReverse Transcription PCR (RT-PCR)
Posted on November 9, 2023
What are the disadvantages of end-point RT-PCR?
PCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
What are the disadvantages of end-point RT-PCR?
PCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on November 8, 2023
What are the advantages of end-point reverse transcription polymerase chain reaction (RT-PCR)?
PCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
What are the advantages of end-point reverse transcription polymerase chain reaction (RT-PCR)?
PCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on November 8, 2023
What are the major differences between qPCR and conventional PCR?
PCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the major differences between qPCR and conventional PCR?
PCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on August 29, 2023
What are the steps of a molecular beacon RT-PCR assay?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyes
What are the steps of a molecular beacon RT-PCR assay?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyes
Posted on August 14, 2023
Why is thermostable DNA polymerase required for PCR?
DNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Why is thermostable DNA polymerase required for PCR?
DNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on August 14, 2023
What are the applications of PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the applications of PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on August 14, 2023
What does Taq polymerase do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What does Taq polymerase do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on August 14, 2023
What do I do if my PCR isn't working?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What do I do if my PCR isn't working?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on August 14, 2023
What does Taq buffer do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What does Taq buffer do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on August 14, 2023
What do forward and reverse primers do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What do forward and reverse primers do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on August 14, 2023
What are the similarities between forward and reverse primers?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the similarities between forward and reverse primers?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on August 14, 2023
What are the steps involved in getting the reverse primer sequence?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the steps involved in getting the reverse primer sequence?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on August 14, 2023
What are the guidelines should I follow when designing primers?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the guidelines should I follow when designing primers?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on August 14, 2023
Why is it necessary to purify PCR products?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Why is it necessary to purify PCR products?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on August 14, 2023
When should I use dPCR to quantify NGS libraries?
DNA and RNA QuantitationPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyesgene sequencing
When should I use dPCR to quantify NGS libraries?
DNA and RNA QuantitationPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyesgene sequencing
Posted on August 3, 2023
What is removed during mRNA processing?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
What is removed during mRNA processing?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on August 3, 2023
How is transcribed mRNA processed in eukaryotes?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
How is transcribed mRNA processed in eukaryotes?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on May 20, 2023
Is magnesium chloride an acid or base?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Is magnesium chloride an acid or base?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 20, 2023
Is magnesium chloride soluble in organic solvents?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)magnesium
Is magnesium chloride soluble in organic solvents?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)magnesium
Posted on May 20, 2023
How does magnesium concentration affect PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
How does magnesium concentration affect PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 20, 2023
What is the ideal concentration of MgCl2?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What is the ideal concentration of MgCl2?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 20, 2023
What is the mechanism of MgCl2 during PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What is the mechanism of MgCl2 during PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 20, 2023
Why is isothermal amplification not as commonly used as PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Why is isothermal amplification not as commonly used as PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 20, 2023
When should I use isothermal amplification instead of PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
When should I use isothermal amplification instead of PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 20, 2023
What are the advantages of isothermal amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the advantages of isothermal amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 20, 2023
What are the differences between isothermal amplification and PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the differences between isothermal amplification and PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 20, 2023
What are the alternative methods of polymerase chain reaction (PCR)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the alternative methods of polymerase chain reaction (PCR)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 12, 2023
What are the similarities between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCRgene sequencing
What are the similarities between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCRgene sequencing
Posted on May 12, 2023
What are the differences between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
What are the differences between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
Posted on May 12, 2023
How does Real-Time PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
How does Real-Time PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
Posted on May 12, 2023
What are the advantages of Real-time PCR over PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
What are the advantages of Real-time PCR over PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
Posted on May 12, 2023
What is the workflow of real-time PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
What is the workflow of real-time PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
Posted on May 12, 2023
What are the methods used to measure the DNA application in qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
What are the methods used to measure the DNA application in qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
Posted on May 12, 2023
Should I use probe-based detection or dye-based detection for my qPCR analysis?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
Should I use probe-based detection or dye-based detection for my qPCR analysis?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
Posted on May 12, 2023
What are the factors I should consider to get accurate and reproducible results for my probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the factors I should consider to get accurate and reproducible results for my probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 12, 2023
What are the advantages of probe-based real-time PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the advantages of probe-based real-time PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 12, 2023
What is the principle of probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What is the principle of probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 12, 2023
How do I design primers for qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
How do I design primers for qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 12, 2023
What should I consider when I set up a PCR efficiency test for my assay?
DNA and RNA QuantitationDNA and RNAPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What should I consider when I set up a PCR efficiency test for my assay?
DNA and RNA QuantitationDNA and RNAPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 12, 2023
What is the gold standard for gene quantification?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What is the gold standard for gene quantification?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 12, 2023
What's the significance of reference genes?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing
What's the significance of reference genes?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing
Posted on May 12, 2023
What is the significance of the cycle threshold?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
What is the significance of the cycle threshold?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
Posted on May 12, 2023
What are the advantages of multiplexing techniques in real time PCR (qPCR)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the advantages of multiplexing techniques in real time PCR (qPCR)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 12, 2023
What factors should I consider when I choose fluorophore/quencher combinations for my multiplex qPCR reactions?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What factors should I consider when I choose fluorophore/quencher combinations for my multiplex qPCR reactions?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 12, 2023
What are the steps involved in probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
What are the steps involved in probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
Posted on March 1, 2023
What is the end product of transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPolymerase Chain Reaction (PCR)PolymerasesReverse Transcription PCR (RT-PCR)
What is the end product of transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPolymerase Chain Reaction (PCR)PolymerasesReverse Transcription PCR (RT-PCR)
Posted on March 1, 2023
What are the differences between cDNA and genomic DNA library?
DNA and RNA QuantitationDNA and RNAGenomic DNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
What are the differences between cDNA and genomic DNA library?
DNA and RNA QuantitationDNA and RNAGenomic DNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on March 1, 2023
What is the significance of cDNA?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
What is the significance of cDNA?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on March 1, 2023
What is the workflow of cDNA library construction?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
What is the workflow of cDNA library construction?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on March 1, 2023
What are the advantages of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
What are the advantages of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on March 1, 2023
What are the disadvantages of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
What are the disadvantages of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on March 1, 2023
What are the steps to construct a genomic DNA library?
DNA and RNA QuantitationDNA and RNAEnzymesGenomic DNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
What are the steps to construct a genomic DNA library?
DNA and RNA QuantitationDNA and RNAEnzymesGenomic DNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on March 1, 2023
What are the applications of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
What are the applications of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on March 1, 2023
What is the procedure of expression screening?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Western Blot
What is the procedure of expression screening?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Western Blot
Posted on March 1, 2023
Where does the transcription terminate?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)PolymerasesReverse Transcription PCR (RT-PCR)
Where does the transcription terminate?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)PolymerasesReverse Transcription PCR (RT-PCR)
Posted on March 1, 2023
Are enhancers necessary for transcription?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Are enhancers necessary for transcription?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on March 1, 2023
What are the promoter sequences?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
What are the promoter sequences?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on March 1, 2023
What are the common primers used in reverse transcription?
DNA and RNA QuantitationDNA and RNAPCR primerPeptide and OligonucleotidePolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)
What are the common primers used in reverse transcription?
DNA and RNA QuantitationDNA and RNAPCR primerPeptide and OligonucleotidePolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)
Posted on March 1, 2023
What are the reaction components for reverse transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)bufferdNTPsinhibitor
What are the reaction components for reverse transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)bufferdNTPsinhibitor
Posted on March 1, 2023
How do I optimize my reverse transcription process?
DNA and RNA QuantitationDNA and RNAGenomic DNANucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
How do I optimize my reverse transcription process?
DNA and RNA QuantitationDNA and RNAGenomic DNANucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on February 23, 2023
What are the differences between CDS and cDNA?
DNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)
What are the differences between CDS and cDNA?
DNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)
Posted on February 23, 2023
What are the applications for reverse transcriptase?
DNA and RNA QuantitationDNA and RNAPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
What are the applications for reverse transcriptase?
DNA and RNA QuantitationDNA and RNAPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)
Posted on February 23, 2023
What is the function of reverse transcriptase?
DNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)
What is the function of reverse transcriptase?
DNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)
Posted on February 23, 2023
How does reverse transcriptase work?
DNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)
How does reverse transcriptase work?
DNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)
Posted on February 17, 2023
What are the differences between Taq polymerase and DNA polymerase?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the differences between Taq polymerase and DNA polymerase?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on February 17, 2023
What are the properties of Taq polymerase enzymes?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the properties of Taq polymerase enzymes?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on February 17, 2023
What is the optimal magnesium concentration for Taq polymerase?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)magnesium
What is the optimal magnesium concentration for Taq polymerase?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)magnesium
Posted on February 17, 2023
What happens if I use higher primer concentrations?
PCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What happens if I use higher primer concentrations?
PCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on February 17, 2023
What is the typical concentration for dNTPs?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What is the typical concentration for dNTPs?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on February 17, 2023
Does PCR use DNA or RNA primers?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Does PCR use DNA or RNA primers?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on February 17, 2023
What are the properties of a primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the properties of a primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on February 13, 2023
What are the steps of PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the steps of PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on February 13, 2023
What are the components required for PCR reaction?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the components required for PCR reaction?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on February 13, 2023
How should oligos be purified for PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
How should oligos be purified for PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on February 13, 2023
How does PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
How does PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on February 13, 2023
What is PCR used for?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What is PCR used for?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on January 18, 2023
How much DMSO do I add to PCR?
DMSODNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
How much DMSO do I add to PCR?
DMSODNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on September 26, 2022
What are the differences between qPCR and ddPCR?
Cell Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological ProbesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the differences between qPCR and ddPCR?
Cell Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological ProbesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on March 3, 2022
What are the differences between a standard PCR and RT PCR?
Reverse Transcription PCR (RT-PCR)Polymerase Chain Reaction (PCR)Chemical ReagentsClassic DyesDNA and RNA Quantitation
What are the differences between a standard PCR and RT PCR?
Reverse Transcription PCR (RT-PCR)Polymerase Chain Reaction (PCR)Chemical ReagentsClassic DyesDNA and RNA Quantitation
Posted on October 25, 2021
Does RNA polymerase need a primer?
Reverse Transcription PCR (RT-PCR)DNA and RNANucleusPhysiological Probes
Does RNA polymerase need a primer?
Reverse Transcription PCR (RT-PCR)DNA and RNANucleusPhysiological Probes
Posted on December 5, 2020
What is reverse transcription PCR?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What is reverse transcription PCR?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on November 23, 2020
What is PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What is PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on June 22, 2020
Are all DNA molecules protein-coding?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)ProteinRNAReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Are all DNA molecules protein-coding?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)ProteinRNAReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on June 22, 2020
What are the general steps of transcription?
DNA and RNA QuantitationDNA and RNAPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What are the general steps of transcription?
DNA and RNA QuantitationDNA and RNAPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on May 9, 2020
What are PCR primers?
Antibodies and ProteomicsDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)qPCR
What are PCR primers?
Antibodies and ProteomicsDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)qPCR
Posted on May 9, 2020
Why am I getting multiple bands for my PCR results?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing
Why am I getting multiple bands for my PCR results?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing
Posted on May 9, 2020
How does next-generation sequencing (NGS) work?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing
How does next-generation sequencing (NGS) work?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing
Posted on April 24, 2020
What is end-point RT-PCR?
Chemical ReagentsClassic DyesDNA and RNA QuantitationDNA and RNAFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)ethidium bromide
What is end-point RT-PCR?
Chemical ReagentsClassic DyesDNA and RNA QuantitationDNA and RNAFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)ethidium bromide
Posted on April 7, 2020
6-VIC, SE is a popular amino-reactive fluorescent probe that is widely used in nucleic acid sequencing and related research. Is it also often used for labeling oligos?
Peptide and Oligonucleotide LabelingDNA sequencingPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
6-VIC, SE is a popular amino-reactive fluorescent probe that is widely used in nucleic acid sequencing and related research. Is it also often used for labeling oligos?
Peptide and Oligonucleotide LabelingDNA sequencingPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)